Abstract : Objective:The present work aims to develop and validate a simple, specific, and accurate reverse phase high performance liquid chromatographic method for the simultaneous estimation of Amlodipine Besylate , Losartan Potassium and Hydrochlorothiazide. Method:The method was developed and validated using Inertsil C8 column(150mm x 4.6 mm, 5μ), with mobile phase consisting of acetonitrile: buffer (700: 500 v/v) with flow rate of 1.0 m l/minute (UV detection 254 nm). Results:The retention times of Amlodipine Besylate , Losartan Potassium and Hydrochlorothiazide were found to be 3.32 min, 2.57 min and 1.92 min respectively. Linearity was observed over a wide concentration range with fairly good sensitivity of 25 -75 μg/ml for Amlodipine Besylate (R2=0.9997), 249-747 μg/ml for Losartan Potassium (R2=0.9991) and 62-188 μg/ml for Hydrochlorothiazide (R2=1.000).The percentage recoveries of Amlodipine Besylate, Losartan Potassium and hydrochlorothiazide were found to be in the range of 99 -101%, 98%-100 % & 98-101% respectively. Conclusion:The proposed method is rapid, accurate and can be used for the routine analysis of Amlodipine Besylate , Losartan Potassium and Hydrochlorothiazide in their formulations.